Part:BBa_M11422:Design

Streptomyces Shuttle Vetor Apra Resistance

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5689
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5695
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5689
  Illegal XhoI site found at 2255
  Illegal XhoI site found at 3905
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5689
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5689
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5704
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2844
  Illegal NgoMIV site found at 2854
  Illegal NgoMIV site found at 2980
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal SapI site found at 786
  Illegal SapI site found at 891
  Illegal SapI site found at 949
  Illegal SapI site found at 3848
  Illegal SapI.rc site found at 640
  Illegal SapI.rc site found at 2951
  Illegal SapI.rc site found at 3322
  Illegal SapI.rc site found at 5456
  

Design Notes

This is a shuttle vector for Streptomyces.

Source

This originated from the plasmid pSET152. Site-directed mutagenesis was used to remove specific restriction sites to make it biobrick compatible.